Job Description

Job Title: Scientific - Associate Research Scientist

Location: Seattle, WA

Duration: 12 Months

Pay Rate: $50.97 - $56.97/ hr on W2

100% Onsite!

Work Schedule- Standard Shift Hours; M-F

Job Description:

The Sequencing and Automation team, part of the Molecular Innovation and Discovery group within Cell Therapy R&D, is seeking a molecular biologist with next generation sequencing experience to fill a one-year contract position. The successful candidate will support efforts to molecularly characterize cell therapy products in various stages of development through our collaborative efforts with the broader research organization. The position will require efforts with experimental design, execution, data collection, data analysis, and presentation of results in team meetings. This role provides great opportunities not only to grow subject matter expertise but also to interact with multiple project teams and cross-functional stakeholders.

Education:

• BS/MS in Biology, Genetics, Molecular Biology or a related field with 1-5 years experience in an academic/industry laboratory.

Duties and Responsibilities:

• Demonstrated ability to effectively work in cross functional team, meet deadlines and prioritize multiple projects.
• Perform DNA/RNA extraction along with appropriate QC methods.
• Develop and optimize molecular assays such as qPCR or ddPCR.
• Make NGS libraries or oversee outsourcing as needed.
• Help develop novel NGS methods to better characterize cell therapy products.
• Order and maintain supplies necessary to support your work or the work of the team.
• Operate and Maintain Sequencers.
• Operate liquid handlers for more routine/higher throughput methods
• Maintain an accurate and detailed electronic notebook
• Compile results into Powerpoint presentations for group meetings

Required skills:

• Experience with DNA/RNA extraction.
• Experience with molecular assay development (qPCR/ddPCR).
• Experience with routine NGS methods. WGS, Fragment libraries, RNASeq, MethylSeq, targeted sequencing etc.

Preferred skills:

• Experience with long read library preparation and sequencing methods (PacBio or Oxford Nanopore).
• Experience with single cell genomics methods (10X Genomics, Parse Biosciences, etc.).
• Experience with lab automation or operation of liquid handlers.
• Familiarity with lentivirus or AAV biology/genomics, as well as T cell or CAR-T biology.
• Experience aseptic technique, cell handling and cell culture
• Experience or interested in learning NGS data analysis or scripting

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